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Strain development

  "Release sterile male-only can increase the effevtive of pest control using sterile insect technique."

         The sterile insect technique will be much more effective when the sterile males are totally separated from the females before the release. It is because the females can reduce the effectiveness of SIT. The females, although sterile, still damage the fruits or transmit diseases and reduce the sterile mating efficacy of the sterile males. The genetic sexing strains (GSSs) allow the mass-separation of the male and female sexes at the early stages. The staffs of RCIB has developed, evaluated, and tested at field trials in the Salaya series of genetic sexing strains based on brown-white pupal color. These strains were developed based on the genetic selection and breeding of natural key fruit fly pest species. The application of them resulted in the reduction of the fruit fly population year round; lower the chemical insecticide usage; higher fruit yield and low infested fruits. The fruit commodity can be leveraged a new international standard in new fruit premium markets which associated with the low prevalence or fruit fly free zone.

Strain development Strain development


Aketarawong N, Isasawin S, Laohakieat K, Thanaphum S* (2020) Genetic stability, genetic variation, and fitness performance of the genetic sexing Salaya1 strain for Bactrocera dorsalis, under long-term mass rearing conditions. BMC Genetics, 21, 131.

Isasawin S, Aketarawong N, Lertsiri S, Thanaphum S* (2014) Development of a genetic sexing strain in Bactrocera carambolae (Diptera: Tephritidae) by introgression of sex sorting components from B. dorsalis, Salaya1 strain. BMC Genetics, 15(Supple 2), S2.

Isasawin S, Aketarawong N, Thanaphum S* (2012) Characterization and evaluation of microsatellite markers in a strain of the oriental fruit fly, Bactrocera dorsalis (Diptera: Tephritidae), with a genetic sexing character used in sterile insect population control. European Journal of Entomology, 109, 331-338.

Contact Details

Regional R&D Training Center for Insect
Biotechnology (RCIB)
Department of Biotechnology, Faculty of Science,
Mahidol University 272 Rama VI Road, Phayathai,
Ratchathewee, Bangkok 10400 THAILAND

Tel: +66 2 441 9820 ext. 1130