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Sex determination

 "Sex determination genes are masters genes for sex differentiation."

         The genetic control program of the sex determination in key fruit fly pests is controlled by a few key genes such as Maleness-of-the-Y (MoY), transformer (tra), transformer-2 (tra-2), doublesex (dsx), fruitless (fru). The basic knowledge of these genes has been used to generate genetic tools. The molecular biotechnology of sex determination pathways has led to a modal sexual-transformation-based genetic sexing system via an ability to masculinize the sterile females. We have investigated sex determination genes in the Bactrocera key pests and undergoing the development of related genetic tools for SIT.


Carraretto D, Aketarawong N, Di Cosimo A, Manni M, Scolari F, Valerio F, Malacrida AR, Gomulski LM, Gasperi G* (2020) Transcribed sex-specific markers on the Y chromosome of the oriental fruit fly, Bactrocera dorsalis. BMG Genetics, 21, 125.

Laohakieat K, Isasawin S, Thanaphum S* (2020) The transformer-2 and fruitless characterisation with developmental expression profiles of sex-determining genes in Bactrocera dorsalis and B. correcta. Scientific Reports, 10, 17938.

Laohakieat K, Aketarawong N, Isasawin S, Thitamadee S, Thanaphum S* (2016) The study of the transformer gene from Bactrocera dorsalis and B. correcta with putative core promoter regions. BMC Genetics 17:34.

Permpoon R, Aketarawong N, Thanaphum S* (2011) Isolation and characterization of Doublesex homologues in the Bactrocera species: Bactrocera dorsalis (Hendel) and B. correcta (Bezzi) and their putative promoter regulatory region. Genetica 139:113-127.

Permpoon R, Thanaphum S* (2010) Isolation and characterization of oligomerization domain I and domain II coding regions of doublesex genes in agricultural fruit flies (Diptera: Tephritidae). European Journal of Entomology 107:121-126.

Contact Details

Regional R&D Training Center for Insect
Biotechnology (RCIB)
Department of Biotechnology, Faculty of Science,
Mahidol University 272 Rama VI Road, Phayathai,
Ratchathewee, Bangkok 10400 THAILAND

Tel: +66 2 441 9820 ext. 1130