DNA markers and population genetics
"Strategy without tactics is the slowest route to victory, tactics without strategy is the noise before defeat." - Sun Tsu, Ancient Chinese military strategy
High-resolution DNA markers systems -such as single nucleotide polymorphisms (SNPs) and microsatellite DNA markers- were developed, evaluated, and used for the studies of molecular systematics, and population dynamics at the levels of macro- and micro- geographies of the key fruit fly pests. These allow the investigation of the colonization, invasion, and species complexity of the fruit fly across their species ranges along with the traceability of infested fruits in global markets. The molecular markers were also effectively used to increase the effectiveness of area-wide integrated pest management using SIT programmes in the mass-rearing facility and in the field operations. Furthermore, Y-pseudo-linked markers were used to unravel the efficacy of reproductive sterility systems for SIT.
Aketarawong N*, Isasawin S, Sojikul P, Thanaphum S (2015) Gene flow and genetic structure of Bactrocera carambolae (Diptera, Tephritidae) among geographical differences and sister species, B. dorsalis, inferred from microsatellite DNA data. In: De Meyer M, Clarke AR, Vera TM, Hendrichs J (Eds): Resolution of Cryptic Species Complexes of tephritid Pests to Enhance SIT Application and Facilitate International Trade. ZooKeys 540(2):239-272.
Manni M, Golmulski LM, Aketarawong N, Gabriella T, Scolari F, Somboon P, Guglielmino CR, Malacrida AR, Gasperi G* (2015) Molecular markers for analyses of interspecific genetic diversity in the Asian Tiger mosquito, Aedes albopictus. Parasites & Vectors 8:188.
Isasawin S, Aketarawong N, Lertsiri S, Thanaphum S* (2014) Development of a genetic sexing strain in Bactrocera carambolae (Diptera: Tephritidae) by introgression of sex sorting components from B. dorsalis, Salaya1 strain. BMC Genetics 15(Supple 2):S2.
Aketarawong N*, Isasawin S, Thanaphum S (2014) Evidence of Weak genetic structure and recent gene flow between Bactrocera dorsalis s.s. and B. papayae, across Southern Thailand and west Malaysia, supporting a single target pest for SIT applications. BMC Genetics 15(1):70.
Aketarawong N, Guglielmino CR, Karam N, Falchetto, Manni M, Scolari F, Gomulski LM, Gasperi G, Malacrida AR* (2014) The oriental fruit fly Bactrocera dorsalis s.s. in East Asia: disentangling the different forces promoting the invasion and shaping the genetic make-up of populations. Genetica 142(3):201-213.
Aketarawong N, Chinvinijkul S, Orankanon W, Guglielmino CR, Franz G, Malacrida AR, Thanaphum S* (2011) The utility of microsatellite DNA markers for the evaluation of area-wide integrated pest management using SIT for the fruit fly, Bactrocera dorsalis (Hendel), control programs in Thailand. Genetica 139:129-140.
Aketarawong N, Bonizzoni M, Thanaphum S, Gomulski LM, Gasperi G, Malacrida AR*, Guglielmin CR (2007) Inferences on the population structure and colonization process of the invasive oriental fruit fly, Bactrocera dorsalis (Hendel). Molecular Ecology 16:3522-2532.
Aketarawong N, Bonizzoni M, Malacrida AR, Gasperi G, Thanaphum S* (2006) Seventeen novel microsatellite markers from an enriched library of the pest species Bactrocera dorsalis sensu stricto. Molecular Ecology Notes 6:1138-1140.